Publisher: Bachudo Science Co. Ltd

Antisickling Activity of Phenylalanine: Effect On Erythrocyte Membrane Atpases

Gloria O. Ibeh, Gabriel I. Ekeke
KEYWORDS: Phenylalanine; sickled cells, erythrocyte membrane ATPases.

ABSTRACT:

in the presence of phenylalanine (Phe). In the presence of 200 µm Phe there was a rapid reversion of already sickled cells. More than forty percent (46.4 ± 3.2. range 42.4 -56.2%) sickling reversion could be achieved within 30 min after the introduction of Phe. A TPase activities were studied in erythrocyte membranes prepared from blood of normal, sickle and heterozygous individuals. Phe stimulated Na+, K+-ATPase activity in both normal and sickle cell nhosts in a concentration dependent manner. Membrane bound Ca2+·ATPase activity in normal and sickle cell ghosts was also stimulated by Phe. Phe inhibited Mg2+-ATPase activity. Inhibition was found to be non-competitive with a ki of 1.9 ± 0.08mM. The antisiclding effect of Phe may be related to its effect on the erythrocyte mernbrane.


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