Publisher: University of Calabar Teaching Hospital

In Vitro Antimicrobial Activity of Titlionia Diversifolia (SUNFLOWER) Leaf Extracts On Aerobic Bacterial isolates From Wound Infection

Ogunjimi T. S., Epoke J
KEYWORDS: In Vitro Antimicrobial Activity of Titlionia Diversifolia (Sunflower) Leaf Extracts on Aerobic Bacterial Isolates from Wound Infection

ABSTRACT:

The in vitro antimicrobial activity of methanol and ethyl acetate extracts ofleafof Tithonia diversifolia on aerobic bacterial isolates from wound infections was studied for medical purpose. Each isolates was identified by relevant biochemical tests.Antimicrobialscreening ofthe two plants extracts was carried out on isolates oiStaphylococcmpygenes, Staphylococcuc aureus, Escherica colt, Klebsiella spp, Proteusspp and Pseudomonas aeruginosa. Astandwd antibioti^ gentamicin was used as positive control. From the sensitivity test performed, both ethyl acetate and metool extracts showed antimicrobial activity against two Grampositive and two Gram negative bacterial isolates (at 2mg/irf) exc^tthe isolates ofProteusspp and Pseudomonas aeruginosa. Ethyl acetate extractshowed wider zones ofinhibitionthanmethMOl extract while gentamicin showed the widest zones ofinhibition. Minimum inhibitory concentration (MIC) values ot the antimicrobial agents on the bacterial isolates that were sensitive to them were also determined using concentottons ranging from 0.78 lug/ml to lOOug/ml. MIC values ofmethanol extract were greater than lOOug/ml whiles the MICs ol gentamicin ethyl acetate extract varied between50ug/ml and ICQ ug/ml. The MICs ofgent^cinvanedbetween 12.5 ug/ml and 25 ug/ml There wasstatistically significant difference between the antibacterial activities ofthe two plant extracts and gentamicin on bacterial isolatesfromwound infections(P<0.05). The MIC values ofethyl acetate extractwere close to Aat ofthe standard antibiotics used in this study. Considering these relatively low MICs recorded in this study, we thus conclude that the organic leafextract ofTithonia diversifolia could be useful as an alternative to orthodox antibiotics mthe treatment ofsome woimd infections.


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