Publisher: Bachudo Science Co. Ltd

Stability of Stored Serum Bilirobin In Calabar, A Tropical Region: Time, Light and Temperature Effect

C. E. J. Udiong, N. I. Kalu, F. I. Ita
KEYWORDS: Bilirubin, Photolability, Correction-chart.

ABSTRACT:

The best storage time for and temperature-dependent photolability of bilirubin in sera were assessed. One hundred pooled Jcteric human sera were divided into four aliquots each, kept under different storage conditions, and analysed for bilirubin at designated intervals by Powel's method of Van der Bergh reaction. Percentage decrease of bilirubin with time was determined for each of the samples stored at 4°C and room temp;rature (as protected and unprotected from light). Storage at 4°C in the dark produced the least and insignificant !ability for total bilirubin up to 3 hours of storage. At room temperature, unconjugated bilirubin was more labile than cQJ1jugated bilirubin, but the reverse was true at 4°C. Temperature-dependent photolability was evident as samples exposed to both light and higher temperature (25°C) showed significant decrease (p < 0.01) in both bilirubin fractions at 15 minutes of storage whilo thoso exposod to light or highor tomJ>oraturo produced significant docrooso ol 30 minutes. It is concluded that ·serum bilirubin is best stored in the dark and at low temperature. A chart to correct for effects of time, light and temperature emanating from delay between blood sampling and analysis for bilirubin in Calabar and similar geographical locations is provided. Such correction would enhance reliance on bilirubin results in monitoring icteric patients.


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