Publisher: Bachudo Science Co. Ltd

Antagonistic Activity of Species of Pseudomonas Against Botryodiplodia Theobromae Pat. In Culture

E. C. Wokoma
KEYWORDS: Antagonistic, Botryodiplodia theobromae, standard biocontrol Pseudomonas.


Fourteen strains of Pseudomonas that produced yellowish-green diffusible pigments on King's medium B were screened for ability to inhibit growth of Botryodiplodia theobromae on Yeast Extract Sucrose Mineral salts (YESM) broth. Mycelial disks of B. theobromae were introduced into Petri dishes containing YESM broth 48 h after the medium was inoculated with bacterial cells. These were compared with standard biocontrol pseudomonads, Pseudomonas fluorescens Pf-5, and P. putida W4P5. Mycelial dry weight determined 72 h after growing the fungus in YESrJ-bacterial culture revealed that the growth of the fungus was significantly reduced by over 50 % in the presence of some of the bacterial strains. Three strains, classified as strong antagonists, were comparatively antagonistic as P. fluorescens Pf-5. Six strains were moderate antagonists, with their antagonistic ability not being significantly different from that of P. putida W4P5. Two strains were weak antagonists. Three strains did not show any antagonistic action against the growth of B. theobromae in culture.

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