Publisher: College of Education, Akamkpa

Effects of Different Concentrations of Polyethylene Glycol On Cells of Three Soybean Cultivars

Uduak M. Afangideh, Donatus F. Uwah
KEYWORDS: Soybean cultivars, Polyethylene glycol (PEG). drought stress, growth rate, total protein.


The responses of three cultivars of Soybean, Glycine max {L) Merr to water stress was studied using different concentrations of Polyethylene glycol (PEG) namely: 0%, 10% and 15 % for 7 and 14 days. Results showed that after a 7 day exposure of the cells of the cultivars to the stress agent, the fresh weight of cultivars Tracy, Hill and Forest were the same in the cootrol groups and the experimentai groups with an average of 1.5810g for the control , 1.5310g and 1.50029 for the 10% and 15% PEG treatments respectively. This indicates that growth rate in the experimental groups for the three cultivars was not affected by the PEG treatments after 7 days of growth. After 14 days of exposure, the fresh weight results for cultivar Hill showed that from an initial fresh weight of 6.27719, fresh weight of the control increased to 1.63759, while that of 10% and 15% PEG treatments increased to 0.9650g and 0.8700g respectively. This low growth rate in the 10% and 15% PEG treatments was the same in all'the cultivars after 14' days exposure to the stress agent. Total protein measurements of cultivar Tracy after 14 days of growth increased from 681.3ug/g cell in control to 932.60ug/gcell and 946.72ug/gcetl for the 10% and 16% PEG treatments. This pattern indicated that there was an increase in protein synthesis when the cells were exposed to longer periods of stress.

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