Publisher: Bachudo Science Co. Ltd

isolation of 2-Methoxyethanol Degrading Bacterial isolate Pseudomonas Sp. Strain Vb.

Frederick Osaro Ekhaise
KEYWORDS: 2-methoxyethanol, environmental samples, degradation, 16S rRNA and Pseudomonas sp. strain VB

ABSTRACT:

Four bacterial isolates from four different environmental samples (agricultural soil, compost soil and sewage sluclge- anaerobic and aerobic wastewaters) were screened for their ability to mineralize 2-methoxyethanol under aerobic conditions. Isolate from anaerobic sew;1ge wastewater was most efficient with an average of 48.0rnmol/day mineralisation capacity. Morphological, physiological, biochemical and molecular characterization of the isolate showed that the strain, designated VB is of the genus Pseudomonas. 16S rRNA sequence analysis sh,.:iwed that the organism is related to Pseudomonas putida at 99.9°.t and Pseudomonas plecog/ossicida at 99.8% similarity level. It is related to members of the genus Pseudomomas belonging to the rRNA group 1 within the gamma M Proteobacteria. The G+C, content 64.5±0.8mol% is within the range characteristic of the genus Pseudomonas.   


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