© Journal Of Applied Sciences And Environmental Management . 2014. Vol. 18 No. 4
Publisher: National Agricultural Research Projects
Evaluation of Antimicrobial Activities of isolated Compounds From The Leaf of The White Specie of Sesamum Indicum From Benue State, Nigeria
Christiana .e. Ogwuche, Joseph .o. Amupitan
KEYWORDS: Anti-microbial, Benue, Pathogenic micro-organisms, Sesamum indicum leaves, Dry vacuum liquid chromatography (DVLC).
ABSTRACT:The isolated compounds from the petroleum spirit, chloroform and methanol crude leaf extracts of the white specie of the leaf of the Sesamum indicum used by traditional medicinal practitioners for the management of infecticious diseases were investigated for in vitro antimicrobial activity against some organisms. The various extracts afforded compounds which had significant antimicrobial activities. The antimicrobial screening showed that all the pure isolates from the different solvent extracts were active against the organisms, Staphylococcus aureus, Streptococcus pyogenes, Salmonella typhi, Candida albicans, Candida krusei, and Candida tropicalis at various MIC’s and (MBC/MFC)’s. The pure isolates from methanol and ethyl acetate fractions were all inactive against Bacillus cereus, Corynebacterium ulcerans, and Escherichia coli. Only the pure isolates from petroleum spirit and chloroform fractions showed activity against Bacillus cereus, Corynebacterium ulcerans, and Escherichia coli at an MIC of 0.5mg/ml and MCB/MFC of 1mg/ml. Chromatographic techniques such as analytical TLC, Purification by dry vacuum liquid chromatography (DVLC) and Preparative TLC were used.
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