© Global Journal Of Pure And Applied Sciences . 2007. Vol. 13 No. 3
Publisher: Bachudo Science Co. Ltd
Surfactant Properties of An Exopolymeric Substance Produced By A Glucose and Hydrocarbon-Utilizing Bacterium isolated From A Brackish Environment In The Niger Delta
G. O. Abu, A. S. Eze
KEYWORDS: surfactant, exopolymenc substance. hydrocarbon utilitzation, surface tension
ABSTRACT:A bacterium was isolated from water samples collected from the New Calabar river in Port Harcourt in the Niger Delta region of Nigeria It was identified as a Bacilus sp based on phenotypic physiological and biochemical characteristics Surfactant property of an exopolymenc substance produced by the isolate was tested using the emulsification of crude oil and surface tension reduction methods The isolate grew in crude Oii (Bonny light and Bonny medium) and glucose as sole sources of carbon; the spent culture filtrates of the crude 01) and the glucose media emulsified the crude oil Further more the surface tension of the glucose ennched culture broth was redu.ced by up to 40%. The biomolecule was precipitated. from culture supernatants as an exocellular polymeric substance, using cold ethanol and further extracted in a 1 1 mixture of chloroform and water, where it collected at the interface, and was shown to contain protein, carbohydrate and lipid The critical micelle concentration (CMG) of the extract was determined to be 0 2gl1 As a further check a Bacillus sp that had been isolated from a soil sample was tested for ability to degrade hydrocarbons The soil isolate did not degrade hydrocarbons. it did not produce an exocellular polymeric substance and the surface tension of the growth media did not change even though ,t grew luxuriantly in glucose enriched medium. as did the nver isolate It is concluded that the river isolate which ,s able to utilize glucose and petroleum hydrocarbons has potenltal application in b1oremed1abon of Oiipolluted environments
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