Publisher: Nigerian Society For Microbiology

Remediation of Used Engine Oil Contaminated Soil By Fungal isolates From Mechanic Workshop Soils

P. G. Olannye, K. I. Ogru, A. O. Emoghene
KEYWORDS: Mycoremediation,Used Engine Oil Contaminated Soil, Biodegradation Potential and Mycoflora

ABSTRACT:

The potential of soil mycoflora isolated from four mechanic workshops, inremediating used engine oil contaminated soil was investigated. Fungal bioload of the soil samples were isolated and enumerated using serial dilution and pour plate methods.; Trichoderma spp., Fusarium spp., Aspergillusniger, Aspergillusflavus, Mucor spp., Rhizopusstolonifer, Penicilliumchrysogenum, Penicillium spp., Aspergillus spp., Saccharomyces spp., Geotrichum spp., Rhizopusstolonifer, Cladosporium spp. and Rhizopusoryzae, with Aspergillusflavus occurring most frequently. Soil samples were observed to be acidic having pH of 6.47 - 6.81 and sandy (50.00%-58.30%). Shake flask degradation test screening indicated that Trichoderma spp., Fusarium spp., Aspergillusniger, Aspergillusflavus, Mucor spp., Rhizopusstolonifer, Penicilliumchrysogenum, Penicillium spp., were able to utilize Hydrocarbon maximally as sole carbon and energy source. Decrease in pH (7.0 – 5.1) and increase in turbidity (0.08-0.90) during the 25 day incubation period was observed during the growth profile result obtained for Aspergillusflavus. Amongst the test organisms used as treatments for hydrocarbon utilization, Aspergillusflavus, consortium of Aspergillusflavus+ Trichoderma spp and consortium of Aspergillusflavus+ Trichoderma spp + Fusarium spp caused the highest percentage reduction of total hydrocarbon content of used engine oil contaminated soil after eight weeks of hydrocarbon utilization. Soils from mechanic workshops are good sources of hydrocarbon utilizers



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